Salycilic Acid Induces Exudation of Crocin and Phenolics in Saffron Suspension-Cultured Cells

Crocin Bleaching Assay Using Purified Di-gentiobiosyl Crocin (-crocin) from Iranian Saffron

OBJECTIVES Crocin bleaching assay (CBA) is a new method for determination of antioxidant capacity. In CBA, addition of hydrogen to the conjugated double bonds of crocin results in reduction of crocin and increase in the absorbance at 440 nm, which is considered as a measure of antioxidant potential. Here CBA method was set up using di-gentiobiosyl crocin or α-crocin from Iranian saffron. Then, ...

Intracellular Localization of Lunularic Acid and Prelunularic Acid in Suspension Cultured Cells of Marchantia polymorpha.

Intracellular localization of lunularic acid and prelunularic acid in suspension cultured cells of Marchantia polymorpha L. was studied. The sum of both compounds was determined as lunularic acid group (LNAs) because of the instability of prelunularic acid to convert into lunularic acid.Mechanical disruption of the cells followed by differential centrifugation showed that LNAs was associated wi...

Selenium elicitation and increase of secondary metabolites production by suspension-cultured Astragalus verus cells

Secondary metabolites with low molecular weight and high economic, pharmaceutical and industrial values play an important role in protecting plants against biotic and abiotic stresses. Astragalus is a medicinal herb, rich in secondary metabolites. Selenium (Se) is an essential element for humans, animals, many bacteria, and a beneficial element for plants. The plants response to selenium is dep...

The effect of virulence on cultivation of Theileria annulata strains in lymphoid cells which have been cultured in suspension

Elicitation of suspension-cultured tomato cells triggers the formation of phosphatidic acid and diacylglycerol pyrophosphate.

Phosphatidic acid (PA) and its phosphorylated derivative diacylglycerol pyrophosphate (DGPP) are lipid molecules that have been implicated in plant cell signaling. In this study we report the rapid but transient accumulation of PA and DGPP in suspension-cultured tomato (Lycopersicon esculentum) cells treated with the general elicitors, N,N',N",N"'-tetraacetylchitotetraose, xylanase, and the fla...